Hamilton O. Smith, Who Made a Biotech Breakthrough, Is Dead at 94

A Nobel laureate, he identified an enzyme that cuts DNA, laying the groundwork for milestones in scientific research and ...
The Scientist

How Restriction Enzymes Changed Biology

Scientists first observed the biological phenomenon of restriction and modification in the 1950s, but they did not identify and isolate restriction enzymes until the late 1960s. 3 In 1970, Hamilton ...
Nature

Restriction Enzymes

In the early 1950s, experiments by two teams of researchers, Salvador Luria working with Mary Human and Joe Bertani working with Jean Weigle, showed that some strains of bacteria were more resistant ...
Nature

DNA restriction-modification enzymes articles from across Nature Portfolio

DNA restriction-modification enzymes are enzymes involved in DNA restriction modification. They include DNA restriction endonucleases (restriction enzymes) and their companion modification methylase ...
JSTOR Daily

How the BfiI Restriction Enzyme Uses One Active Site to Cut Two DNA Strands

Unlike other restriction enzymes, BfiI functions without metal ions. It recognizes an asymmetric DNA sequence, 5′-ACTGGG-3′, and cuts top and bottom strands at fixed positions downstream of this ...
JSTOR Daily

Construction and evaluation of a transformant library of Lasiodiplodia theobromae generated through Restriction Enzyme-Mediated Integration

Grapevine dieback, caused by Lasiodiplodia theobromae, is an important trunk disease worldwide. Transformants of L. theobromae were generated in an attempt to identify potential pathogenicity-related ...